Locate the therapy transducer focus
Prior to the treatment, a bubble cloud was generated in an empty water bath and showed as hyperechoic zone on B-mode ultrasound image. This hyperechoic zone was marked as the therapy transducer focus.
Verification of the target localization in the in vivo liver tissue
After the therapy focus marker with aligned with the targeted liver volume, a small number of histotripsy pulses were applied to create a bubble cloud in the liver tissue, which showed as a hyperechoic zone.